Difference between revisions of "Part:BBa I746001"

Revision as of 07:17, 23 October 2007

I746000 (AIP generator - agrB + agrD with RBSes) + B0015 (Terminator)

This part is simply the AIP generator from the agr system with a standard registry terminator (B0015)downstream.

The original (I746000) part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.

In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.

This part contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_I746001 short</partinfo>
-This part is simply the AIP generator from the <i>agr</i> system with a standard registry terminator downstream.
+This part is simply the AIP generator from the <i>agr</i> system with a standard registry terminator ([https://parts.igem.org/wiki/index.php/Part:BBa_B0015 B0015])downstream.
 The original ([https://parts.igem.org/wiki/index.php/Part:BBa_I746000 I746000]) part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.