Latest revision as of 11:07, 26 October 2017

Introduction

Modified Gα Schizosaccharomyces pombe

Gα plays an important role in MAPK pathway. In the yeast endogenous metabolic pathway, the conformation of Ste2 changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. In order to make the sweetness receptor fit the Gα better, we modified the Gα. Then the sweetness receptor can combine with the Gα and send the signal to the downstream.

Fig.1 The structure of Gα in Schizosaccharomyces pombe

Design

Mutated C-terminus 15bp sequence of the yeast Gα, and replace it with the Schizosaccharomyces pombe Gα subunit. After construct the modified Gα by PCR, and the initial sequence is amplified from Saccharomyces Cerevisiae CEN.PK2-1C.

Fig.2 The schematic diagram of Modified Gα-Schizosaccharomyces pombe

Experiment

The part is used to improve the matching condition of sweetness receptor and the modified Gα. The length of sequence is 1421bp.

Fig.3 Electrophoresis of the positive result of the modified Gα- Schizosaccharomyces pombe

Sequence and Features