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===Applications of BBa_K1072023===
 
===Applications of BBa_K1072023===
 
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<h1>BBa_K2368005</h1>
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<h1>Introduction</h1>
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<h2>Introduction</h2>
 
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Latest revision as of 10:45, 26 October 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1072023

BBa_K2368005

Introduction

Pfus

Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae, when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.

However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)

标题 ‎

Fig.1 The sequencing result of Pfus

This part is an improvement of BBa_K1072023 of 2013 SCUT team.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



User Reviews

UNIQc4ccbb3d933784b2-partinfo-00000002-QINU

UNIQc4ccbb3d933784b2-partinfo-00000003-QINU