Difference between revisions of "Part:BBa K2247000"

Revision as of 08:22, 30 October 2017

pGalS promoter of S. cerevisiae

This is a modified version of the yeast endogenous Gal1 promoter, from which expression can be induced by galactose, or the yeast Gal4 activator. pGalS also contains the upstream activating sequence (UAS) which Gal4 can bind, and can serve as the promoter in a standard yeast-two-hybrid system.

Usage and Biology

This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, or simply the addition of galactose, with a higher strength than pGal1. This promoter can serve as an positively regulated promoter in the S. cerevisiae system, or drive the expression of the reporter gene in any standard version of yeast-two-hybrid system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 453
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 54
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, with a higher strength than pGal1.
+This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, or simply the addition of galactose, with a higher strength than pGal1. This promoter can serve as an positively regulated promoter in the S. cerevisiae system, or drive the expression of the reporter gene in any standard version of yeast-two-hybrid system.
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