Difference between revisions of "Part:BBa K2230022"
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=== Research === | === Research === | ||
Based on our research, the glucose transporter of ''Salmonella typhimurium'' has lowest Km, meaning the highest affinity of glucose bound to the transporter | Based on our research, the glucose transporter of ''Salmonella typhimurium'' has lowest Km, meaning the highest affinity of glucose bound to the transporter | ||
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Revision as of 03:58, 26 October 2017
STM1128/pSB1C3
Salmonella typhimurium LT2 has two glucose-specific transporter systems, PTS system and sodium/glucose cotransporter. PTS system contains two subunits IIA encoded by crr and IIBC by ptsG which are assembled to a high-affinity active transporter. The other is a Na+/glucose cotransporter encoded by STM1128 that contributes to facilitated transport with lower glucose affinity. Based on our research, the glucose transporter of Salmonella has a lower Km compared to human small intestine, Staphylococcus and E. coli, indicating a higher efficiency for glucose uptake. In our project, we created the glucose transporter device and genetically engineer microbes with these two systems.
Research
Based on our research, the glucose transporter of Salmonella typhimurium has lowest Km, meaning the highest affinity of glucose bound to the transporter
Cloning
The STM1128 gene was amplified from gDNA of Salmonella typhimurium and cloned onto pSB1C3. This part has been sequenced.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1255
- 1000COMPATIBLE WITH RFC[1000]