Difference between revisions of "Part:BBa K2477002"

Latest revision as of 23:24, 25 October 2017

sgRNA Regulating GFP (E0040) Chromophore

This part includes a base pairing region as well as a handle for interaction with Cas9 and a terminator. The base pairing region overlaps the chromophore region of GFP (E0040). Specifically, it overlaps base pairs 188 through 207 of E0040. It was designed in order to select against GFP variants lacking any sort of mutation within this base pairing region. It needs to be assembled with a promoter in order to be fully functional. It will then need to be used with BBa_K2477003 in order to select against GFP non-mutants.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2477002 short</partinfo>
-This part includes a base pairing region as well as a scaffold for interaction with Cas9 and a terminator. The base pairing region overlaps the chromophore region of GFP (E0040). Specifically, it overlaps base pairs 188 through 207 of E0040. It was designed in order to select against GFP variants lacking any sort of mutation within this base pairing region. It needs to be assembled with a promoter in order to be fully functional.
+This part includes a base pairing region as well as a handle for interaction with Cas9 and a terminator. The base pairing region overlaps the chromophore region of GFP (E0040). Specifically, it overlaps base pairs 188 through 207 of E0040. It was designed in order to select against GFP variants lacking any sort of mutation within this base pairing region. It needs to be assembled with a promoter in order to be fully functional. It will then need to be used with BBa_K2477003 in order to select against GFP non-mutants.
 <!-- Add more about the biology of this part here