Difference between revisions of "Part:BBa I739203"
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Plasmid pCK01MCS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.<br>
 
Plasmid pCK01MCS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.<br>
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In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], a MCS harbouring a SpeI site was introduced by PCR. This MCS is the only difference between pCKMCS1 and pCK01:<br>
 
In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], a MCS harbouring a SpeI site was introduced by PCR. This MCS is the only difference between pCKMCS1 and pCK01:<br>
 
[[Image:PCK01-2.jpg]]
 
[[Image:PCK01-2.jpg]]
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===Purpose===
 
===Purpose===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Revision as of 20:32, 22 October 2007

No part name specified with partinfo tag.

Plasmid pCK01MCS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.
Pck01 Andy.jpg

In order to make this plasmid compatible with the BioBrick Standard assembly process, a MCS harbouring a SpeI site was introduced by PCR. This MCS is the only difference between pCKMCS1 and pCK01:
PCK01-2.jpg

Purpose

This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and contains...


References

[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000


Sequence and Features No part name specified with partinfo tag.