Difference between revisions of "Part:BBa K2520017"

Revision as of 18:53, 26 October 2017

CMV-Mono Display:PLA 1-hGH

This device is a complete system for displaying proteins on cells' membrane. The Igk leader (BBa_K2520024) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (BBa_K2520026) is a trans-membrane domain that anchors all the components located between the igk leader and the PDGFR itself to the membrane. HA (BBa_K2520030) is a tag that binds to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of bee venom allergy (BBa_K2520045, Phospholipases A2 protein) and is component related to our specific project. The CMV promoter (BBa_K1119006) is a constitutive expression promoter in mammalian cells and hGH (BBa_K404108) serves as a terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 614
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 679
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1390

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2520017 short</partinfo>
-CMV-mono PLA 1-hGH
+This device is a complete system for displaying proteins on cells' membrane. The Igk leader (<partinfo>K2520024</partinfo>) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (<partinfo>K2520026</partinfo>) is a trans-membrane domain that anchors all the components located between the igk leader and the PDGFR itself to the membrane. HA (<partinfo>K2520030</partinfo>) is a tag that binds to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of bee venom allergy (<partinfo>K2520045</partinfo>, Phospholipases A2 protein) and is component related to our specific project.
+The CMV promoter (<partinfo>K1119006</partinfo>) is a constitutive expression promoter in mammalian cells and hGH (<partinfo>K404108</partinfo>) serves as a terminator.
 <!-- Add more about the biology of this part here