Difference between revisions of "Part:BBa K2235009:Experience"
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[[File:SiaComp_1.png|600px|thumb|left|Figure 1]] | [[File:SiaComp_1.png|600px|thumb|left|Figure 1]] | ||
| − | + | From left to right: M DNA ladder, next 2 lanes are plasmids digested with Ecor1 and Pst1. | |
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:36, 25 October 2017
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Molecular cloning
Ligation of sialidase composite insert into pSB1C3
The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the double digestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
From left to right: M DNA ladder, next 2 lanes are plasmids digested with Ecor1 and Pst1.
User Reviews
UNIQ440bf5f3805bdde3-partinfo-00000000-QINU UNIQ440bf5f3805bdde3-partinfo-00000001-QINU