Difference between revisions of "Part:BBa K2368028"
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__NOTOC__ | __NOTOC__ | ||
<h1>Introduction</h1> | <h1>Introduction</h1> | ||
| − | <partinfo>BBa_K2368028 | + | <partinfo>BBa_K2368028 short</partinfo> |
<p> Gα plays an important role in<i> Saccharomyces Cerevisiae CEN.PK2-1C </i>MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream. </p> | <p> Gα plays an important role in<i> Saccharomyces Cerevisiae CEN.PK2-1C </i>MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream. </p> | ||
| − | [[File:Ga-1.png| | + | [[File:Ga-1-HR.png|center|500px|加载失败时候的说明文字]] |
<p style="text-align: center"> Fig.1 The structure of Gα</p> | <p style="text-align: center"> Fig.1 The structure of Gα</p> | ||
<h1>Design</h1> | <h1>Design</h1> | ||
<p> Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.</p> | <p> Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.</p> | ||
| − | [[File: G-1.png| | + | [[File: G-1-HR.png|center|500px|加载失败时候的说明文字]] |
<p style="text-align: center"> Fig.2 The schematic diagram of Modified Gα</p> | <p style="text-align: center"> Fig.2 The schematic diagram of Modified Gα</p> | ||
<h1>Experiment</h1> | <h1>Experiment</h1> | ||
<p> The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.</p> | <p> The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.</p> | ||
| − | [[File: G-2.png| | + | [[File: G-2-HR.png|center|500px|加载失败时候的说明文字]] |
<p style="text-align: center"> Fig.3 The electrophoresis of the positive result of Gα.</p> | <p style="text-align: center"> Fig.3 The electrophoresis of the positive result of Gα.</p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<h2>Sequence and Features</h2> | <h2>Sequence and Features</h2> | ||
<partinfo>BBa_K2368028 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2368028 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2368028 arameters</partinfo> | <partinfo>BBa_K2368028 arameters</partinfo> | ||
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Revision as of 10:22, 26 October 2017
Introduction
Gα- MAPK pathway signal transduction factor
Gα plays an important role in Saccharomyces Cerevisiae CEN.PK2-1C MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream.
Fig.1 The structure of Gα
Design
Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.
Fig.2 The schematic diagram of Modified Gα
Experiment
The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.
Fig.3 The electrophoresis of the positive result of Gα.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]