Difference between revisions of "Part:BBa K2235009:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | '''Ligation of sialidase composite insert into pSB1C3''' | ||
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The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively. | The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively. | ||
Revision as of 17:50, 25 October 2017
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how you used this part and how it worked out.
Molecular cloning
Ligation of sialidase composite insert into pSB1C3
The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
User Reviews
UNIQab6bf0779fb30fe9-partinfo-00000000-QINU UNIQab6bf0779fb30fe9-partinfo-00000001-QINU