Difference between revisions of "Part:BBa K2368015"
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<h1>Design</h1> | <h1>Design</h1> | ||
<p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and GFP. Then, we combined the overlap of T1R3 with the GFP by PCR.</p> | <p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and GFP. Then, we combined the overlap of T1R3 with the GFP by PCR.</p> | ||
| − | [[File:T-BIT-China-2017parts- | + | [[File:T-BIT-China-2017parts-27.png|center|500px|默认文字]] |
<p style="text-align: center">Fig.1 The schematic diagram of GFP+T1R2 overlap</p> | <p style="text-align: center">Fig.1 The schematic diagram of GFP+T1R2 overlap</p> | ||
| − | [[File:T-BIT-China-2017parts- | + | [[File:T-BIT-China-2017parts-28.png|center|500px|默认文字]] |
<p style="text-align: center">Fig. 2 The PCR method of GFP+T1R2 overlap</p> | <p style="text-align: center">Fig. 2 The PCR method of GFP+T1R2 overlap</p> | ||
<h1>Experiment</h1> | <h1>Experiment</h1> | ||
<p>This part is used to detect the expression and the location of the T1R2.</p> | <p>This part is used to detect the expression and the location of the T1R2.</p> | ||
| − | [[File:T-BIT-China-2017parts- | + | [[File:T-BIT-China-2017parts-29.png|center|500px|默认文字]] |
<p style="text-align: center">Fig.3 Electrophoresis of GFP+T1R2 overlap. Line one is the target</p> | <p style="text-align: center">Fig.3 Electrophoresis of GFP+T1R2 overlap. Line one is the target</p> | ||
Revision as of 17:33, 25 October 2017
Introduction
T1R2-GFP
This part is the overlap of that sweetness receptor T1R2 combines with the GFP tag.
The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and GFP tag.
The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.
Design
Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and GFP. Then, we combined the overlap of T1R3 with the GFP by PCR.
Fig.1 The schematic diagram of GFP+T1R2 overlap
Fig. 2 The PCR method of GFP+T1R2 overlap
Experiment
This part is used to detect the expression and the location of the T1R2.
Fig.3 Electrophoresis of GFP+T1R2 overlap. Line one is the target
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]