Revision as of 10:59, 26 October 2017

Introduction

T1R2-YCP

This part is the overlap of that sweetness receptor T1R2 combines with the YCP tag.

The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and YCP tag.

The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.

Design

Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and YCP. Then, we combined the overlap of T1R2 with the YCP by PCR.

Fig.1 The schematic diagram of YCP+T1R2 overlap

Fig. 2 The PCR method of YCP+T1R2 overlap

Experiment

This part is used to detect the expression and the location of the T1R2.

Fig.3 Electrophoresis of YCP+T1R2 overlap. Line one is the target

Sequence and Features