Difference between revisions of "Part:BBa I742103"

Revision as of 12:40, 23 October 2007

pTG262 Plasmid (Gram negative to Gram positive shuttle vector)

Plasmid contains an origin of replication which is capable of replicating in E. coli and several gram positive organisms including Lactobacillus, Lactococcus and Bacillus. Plasmid has chloramphenicol, neomycin & gentamicin resistance genes. It also has a EcoRI and PstI site with an XbaI site situated in the middle so biobricks can be inserted. Insertion of any biobrick between the unique EcoRI and PstI sites removes the only XbaI site, and there are no SpeI sites, so the product is a compliant biobrick. pTG262 was generously provided by Dr. Mike Gasson of the Institute of Food Research, Norwich, UK. Recommended selection with chloramphenicol is as follows: E. coli 15 mg/l; Bacillus subtilis 10 mg/l; Lactococcus lactis 5 mg/l; Lactobacillus spp. 7.5 mg/l.

Gene instability may occur with certain inserts (we have not experienced this so far with the biobricks we have inserted).

For more information about pTG262 visit the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Gene_Expression_In_Lactobacillus Edinburgh Yoghurt Wiki]

Sequence and Features