Difference between revisions of "Part:BBa K2244007"

Revision as of 15:39, 25 October 2017

ColE promoter +mhei gene +mcherry gene +T1terminator

The device is a functional plasmid containing a MBC hydrolase gene (mheI) (BBa_K2244004), which encodes carbendazim hydrolase. In order to study expression of target gene in cytoplasm, mheI was fused to a reported gene mCherry (BBa_J06504), which functions as indicator of the uptake of mheI. This part is regulated under the control of ColE promoter, which is from E. coli ColE1 plasmid.

Biology

ColE promoter(BBa_K2244006) sequence is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.

mCherry(BBa_K2244008) is a red fluorescent protein used as a reporter. It is based on a fluorescent protein that was originally isolated from Discosoma sp.mCherry sequence is codon optimized for E. coli expressionm and can be replaced by any gene sequence in light-regulated studies.

MBC hydrolase gene (mheI)(BBa_K2244004) is derived from carbendazim-degrading strain Mycobacterium sp. SD-4 and is a genomic DNA gene sequence. mheI gene is also evolutionary conserved in many other bacteria strains, such as Nocardioides sp. SG-4G, and Rhodococcus erythropolis.

T1terminator is the most commonly used terminator.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1250
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1250
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 754
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1250
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1250
Illegal AgeI site found at 413
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K2244007 short</partinfo>
 The device is a functional plasmid containing a MBC hydrolase gene (mheI) ([https://parts.igem.org/Part:BBa_K2244004 BBa_K2244004]), which encodes carbendazim hydrolase. In order to study expression of target gene in cytoplasm, mheI was fused to a reported gene mCherry ([https://parts.igem.org/Part:BBa_J06504 BBa_J06504]), which functions as indicator of the uptake of mheI. This part is regulated under the control of ColE promoter, which is from E. coli ColE1 plasmid.
-===
+===Biology===
+ColE promoter([https://parts.igem.org/Part:BBa_K2244006 BBa_K2244006]) sequence is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.
+mCherry([https://parts.igem.org/Part:BBa_K2244008 BBa_K2244008]) is a red fluorescent protein used as a reporter. It is based on a fluorescent protein that was originally isolated from Discosoma sp.mCherry sequence is codon optimized for E. coli expressionm and can be replaced by any gene sequence in light-regulated studies.
+MBC hydrolase gene (mheI)([https://parts.igem.org/Part:BBa_K2244004 BBa_K2244004]) is derived from carbendazim-degrading strain Mycobacterium sp. SD-4 and is a genomic DNA gene sequence. mheI gene is also evolutionary conserved in many other bacteria strains, such as Nocardioides sp. SG-4G, and Rhodococcus erythropolis.
+T1terminator is the most commonly used terminator.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===