Difference between revisions of "Part:BBa K2492004"
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<partinfo>BBa_K2492004 short</partinfo> | <partinfo>BBa_K2492004 short</partinfo> | ||
+ | Part BBa_K2492004 contains odr-10. It is a section of genomic DNA acts as a promoter. | ||
+ | In C.elegans, ODR-10 encodes a large family of seven transmembrane olfactory receptor genes[1]which is specifically expressed in neuron AWA and affect chemotaxis to the volatile diacety. POrd-10 is a section of genemoic DNA in front of Ord-10 genes promoting expression of Ord-10 genes[2]. | ||
+ | AWA is a member of the olfactory neurons [2]. It responses to attractive volatile odorants. Since we expect to express the functional channelrhodopsins and indicator in specific neuron(AWA for instance), we use pODR-10 to construct plasmid. | ||
+ | If CoCHR is expressed in AWA, we are capable of activating it to manipulate the state of AWA. In our project, we integrated ord-10::Chrimson::GEM-GECO::mCherry transgene to C.elegans by microinjection. Thus, CoCHR (Part BBa_K2492001:CoCHR) promoted by ord-10 is expressed in AWA. We can enhance the attraction of C.elegans through the control of AWA. | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2492004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2492004 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K2492004 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 06:25, 29 October 2017
pODR-10: the promoter of coding odorant diacetyl receptor protein.
Part BBa_K2492004 contains odr-10. It is a section of genomic DNA acts as a promoter. In C.elegans, ODR-10 encodes a large family of seven transmembrane olfactory receptor genes[1]which is specifically expressed in neuron AWA and affect chemotaxis to the volatile diacety. POrd-10 is a section of genemoic DNA in front of Ord-10 genes promoting expression of Ord-10 genes[2]. AWA is a member of the olfactory neurons [2]. It responses to attractive volatile odorants. Since we expect to express the functional channelrhodopsins and indicator in specific neuron(AWA for instance), we use pODR-10 to construct plasmid. If CoCHR is expressed in AWA, we are capable of activating it to manipulate the state of AWA. In our project, we integrated ord-10::Chrimson::GEM-GECO::mCherry transgene to C.elegans by microinjection. Thus, CoCHR (Part BBa_K2492001:CoCHR) promoted by ord-10 is expressed in AWA. We can enhance the attraction of C.elegans through the control of AWA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]