Difference between revisions of "Part:BBa K2328000"
(→‎Results)
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==Results==
 
==Results==
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We do many eperiments on smURFP.
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:'''I. Protein production and purification'''
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 +
 +
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:'''II. Protein crystallization'''
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<p style="text-align: center;">
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    https://static.igem.org/mediawiki/parts/c/c6/Cristal.png<br>
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'''Table 1.'''  Result of Microplate Reader in the black 96-well plate. Tube 1 and 2 are experimental group, and tube 3 is the control group.<br>
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 +
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:'''III. Protein and BV'''
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:'''VI. co-expression with HO-1'''
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Plasmid pET28b with smURFP and HO-1 gene were transformed into E. coli BL-21. We used this induced bacteria to confirm the fluorescence and data showed a relatively high value, as shown in table 1.
 +
<p style="text-align: center;">
 +
    https://static.igem.org/mediawiki/parts/e/e5/Microplate_Reader.png<br>
 +
'''Table 1.'''  Result of Microplate Reader in the black 96-well plate. Tube 1 and 2 are experimental group, and tube 3 is the control group.<br>
 +
</p>
 +
 +
Then laser confocal microscopy was use to observe these bacteria, activate light of 640nm was used, as shown in Figure 2.
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 +
<p style="text-align: center;">
 +
    https://static.igem.org/mediawiki/parts/f/f1/Confocal.jpg<br>
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'''Figure 2.'''  The result after induction, the upper one is the control group, and the inferior one is the experimental group.<br>
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</p>

Revision as of 14:20, 25 October 2017


smURFP (I, codon-optimized for Escherichia coli) (without terminator codon TAA)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

Biology

Reference

Results

We do many eperiments on smURFP.

I. Protein production and purification


II. Protein crystallization

Cristal.png
Table 1. Result of Microplate Reader in the black 96-well plate. Tube 1 and 2 are experimental group, and tube 3 is the control group.

III. Protein and BV
VI. co-expression with HO-1
Plasmid pET28b with smURFP and HO-1 gene were transformed into E. coli BL-21. We used this induced bacteria to confirm the fluorescence and data showed a relatively high value, as shown in table 1. <p style="text-align: center;"> Microplate_Reader.png
Table 1. Result of Microplate Reader in the black 96-well plate. Tube 1 and 2 are experimental group, and tube 3 is the control group.

Then laser confocal microscopy was use to observe these bacteria, activate light of 640nm was used, as shown in Figure 2.

Confocal.jpg
Figure 2. The result after induction, the upper one is the control group, and the inferior one is the experimental group.