Difference between revisions of "Part:BBa I742130:Experience"
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===Applications of BBa_I742130=== | ===Applications of BBa_I742130=== | ||
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| + | We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520]. | ||
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| + | It gave a lower fluorescence than our designed reverse RBS [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560]. | ||
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| + | Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator. | ||
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| + | BBa_I742130 translation initiation rate 495.33 | ||
| + | BBa_K1463560 translation initiation rate 12,204.43 | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:45, 16 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I742130
We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick BBa_K1463520.
It gave a lower fluorescence than our designed reverse RBS BBa_K1463560.
Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator.
BBa_I742130 translation initiation rate 495.33 BBa_K1463560 translation initiation rate 12,204.43
User Reviews
UNIQ0a5d055eaafda40e-partinfo-00000000-QINU UNIQ0a5d055eaafda40e-partinfo-00000001-QINU