Difference between revisions of "Part:BBa K2282007"

Revision as of 12:51, 25 October 2017

BBa_K592009 (AmilCP) supplemented with BBa_K2282002 (CspA 5'UTR)

Usage and Biology

AmilCP is a coral chromoprotein which displays a strong blue color. The amilCP expresses a strong color, visible to the naked eye and with a temperature-dependent expression when used with a weak promoter.

Source of this part

The 5’UTR sequence has been taken from the NCBI genebank on the Cold-shock protein A gene sequence. Mitta et al, 1997 clearly indicated its position so we just had to take the sequence from here. Sequences of the 5’UTR, DSBox and other cold-induced elements are very close to the parts of the iGEM10_Mexico-UNAM-CINVESTAV that can be found here : http://partsregistry.org/Part:BBa_K328003

Design and considerations

This part has no RBS because there is one already located on the 5’UTR, which is the Shine-Dalgarno sequence (Yamanaka et al, 1999) : 5’AAGG 3’

Sequence and Features