Difference between revisions of "Part:BBa K2282005"
 
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<partinfo>BBa_K2282005 short</partinfo>
 
<partinfo>BBa_K2282005 short</partinfo>
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AmilCP is expressed thanks to the Anderson constitutive promoter and translation is initiated at the ribosome binding site. AmilCP is a chromoprotein that has already been successfully used by several iGEM teams and leads to a strong color visible to the naked eye (The protein has an absorbance maximum at 588 nm) but also a temperature-dependent expression when used with a weak promoter. The double terminator enables robust termination of transcription.
 
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 12:50, 25 October 2017


BBa_K592009 (AmilCP) consitutive expression system.

Usage and Biology

AmilCP is expressed thanks to the Anderson constitutive promoter and translation is initiated at the ribosome binding site. AmilCP is a chromoprotein that has already been successfully used by several iGEM teams and leads to a strong color visible to the naked eye (The protein has an absorbance maximum at 588 nm) but also a temperature-dependent expression when used with a weak promoter. The double terminator enables robust termination of transcription.

Source of this part

The genomic sequence has been found on the IGEM registry of standard biological parts and the part has been assembled.

Design consideration

None.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]