Difference between revisions of "Part:BBa K2381010"

Revision as of 09:49, 25 October 2017

Cod1+ rigid linker+pMagFast In this part, the light-induced dimerization protein,<a href="https://parts.igem.org/Part:BBa_K2381001">pMag</a>, is fused with the N fragment of split dCas9 through rigid linker.
The alpha-helix structure of the rigid linker render more cooperativity between split dCas9 and dimerization protein.
It's protein sequence is A(EAAAK)3A, the flanking Ala guarantees a flexibility conjunction, which will decrease the effect on the function of protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1099
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2277
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2381010 short</partinfo>
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 In this part, the light-induced dimerization protein,<a href="https://parts.igem.org/Part:BBa_K2381001">pMag</a>, is fused with the N fragment of split dCas9 through rigid linker.<br/>
 The alpha-helix structure of the rigid linker render more cooperativity between split dCas9 and dimerization protein.<br/>
 It's protein sequence is A(EAAAK)3A, the flanking Ala guarantees a flexibility conjunction, which will decrease the effect on the function of protein.
+<!-- Add more about the biology of this part here
+===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>