Difference between revisions of "Part:BBa K2244011"

 
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<partinfo>BBa_K2244011 short</partinfo>
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The device is a functional plasmid containing a light repressor LEV1 ([https://parts.igem.org/Part:BBa_K2244005 BBa_K2244005]), which undergoes conformational change upon light irradiation and dimerized to bind regulatory sequence on ColE promoter ([https://parts.igem.org/Part:BBa_K2244006 BBa_K2244006]), thus inhibits target gene expression. While in darkness, dimerization does not occur thus gene expression proceeds. This device is a light repressing system with high induction efficiency and low leakage. mCherry (([https://parts.igem.org/Part:BBa_K2244008 BBa_K2244008])) is reporter gene used in this system to prove its function as well as help to characterize it. mCherry gene can be replaced by any gene sequence in light-regulated studies
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2244009 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2244009 parameters</partinfo>
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Revision as of 16:11, 25 October 2017


CloE promoter+TorA+opdA+T1 terminator

The device is a functional plasmid containing a light repressor LEV1 (BBa_K2244005), which undergoes conformational change upon light irradiation and dimerized to bind regulatory sequence on ColE promoter (BBa_K2244006), thus inhibits target gene expression. While in darkness, dimerization does not occur thus gene expression proceeds. This device is a light repressing system with high induction efficiency and low leakage. mCherry ((BBa_K2244008)) is reporter gene used in this system to prove its function as well as help to characterize it. mCherry gene can be replaced by any gene sequence in light-regulated studies

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1015
    Illegal NheI site found at 1038
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 845
    Illegal AgeI site found at 1753
  • 1000
    COMPATIBLE WITH RFC[1000]