Difference between revisions of "Part:BBa K2244009"
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<partinfo>BBa_K2244009 short</partinfo> | <partinfo>BBa_K2244009 short</partinfo> | ||
| − | The device is a functional plasmid containing a light repressor LEV1 (BBa_K2244005), which undergoes conformational change upon light irradiation and dimerized to bind regulatory sequence on ColE promoter (BBa_K2244006), thus inhibits target gene expression. While in darkness, dimerization does not occur thus gene expression proceeds. This device is a light repressing system with high induction efficiency and low leakage. mCherry (BBa_K2244008) is reporter gene used in this system to prove its function as well as help to characterize it. mCherry gene can be replaced by any gene sequence in light-regulated studies | + | The device is a functional plasmid containing a light repressor LEV1 ([https://parts.igem.org/Part:BBa_K2244005 BBa_K2244005]), which undergoes conformational change upon light irradiation and dimerized to bind regulatory sequence on ColE promoter ([https://parts.igem.org/Part:BBa_K2244006 BBa_K2244006]), thus inhibits target gene expression. While in darkness, dimerization does not occur thus gene expression proceeds. This device is a light repressing system with high induction efficiency and low leakage. mCherry (([https://parts.igem.org/Part:BBa_K2244008 BBa_K2244008])) is reporter gene used in this system to prove its function as well as help to characterize it. mCherry gene can be replaced by any gene sequence in light-regulated studies |
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Revision as of 15:50, 25 October 2017
ColE promoter+mCherry gene+T1 terminator+Constitutive promoter+Lev1 gene+T1terminator
The device is a functional plasmid containing a light repressor LEV1 (BBa_K2244005), which undergoes conformational change upon light irradiation and dimerized to bind regulatory sequence on ColE promoter (BBa_K2244006), thus inhibits target gene expression. While in darkness, dimerization does not occur thus gene expression proceeds. This device is a light repressing system with high induction efficiency and low leakage. mCherry ((BBa_K2244008)) is reporter gene used in this system to prove its function as well as help to characterize it. mCherry gene can be replaced by any gene sequence in light-regulated studies
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1015
Illegal NheI site found at 1038 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 845
Illegal AgeI site found at 1753 - 1000COMPATIBLE WITH RFC[1000]