Difference between revisions of "Part:BBa K2201207"

Figure 1: Plasmid map of the BioBrick K2201207 coding for a AzoF-tRNA-synthetase system based on the ONBY-Part (K1416000). The ONBY-RS was removed by PCR and then a gene containing the AzoF-RS and matching overlaps sides was integrated through Gibson assembly.

Figure 2: History of the plasmid construction of K2201207.

Figure 3: Alignment of the protein sequence of the AzoF-RS and the M. jannaschii TyrRS.

Figure 4: Structure of AzoF in cis- and trans-conformation. Switching is possible via irradiation.

Figure 5: Absorption spectrum of AzoF in LB media after irradiation with light of 367 nm and 465 nm wavelength. The black line shows the typical absorption of AzoF in a mixed state, the trans-conformation is blue and green and the other lines show the absorption spectrum in the cis-conformation.