Difference between revisions of "Part:BBa K2486021"

 
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<partinfo>BBa_K2486021 short</partinfo>
 
<partinfo>BBa_K2486021 short</partinfo>
  
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Intermediarry assembly between parts BBa_K2486021 and BBa_K2486004
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Biosensor constructed by the ligation of parts BBa_K2486021 and BBa_K2486004.
  
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===Usage and Biology===
 
===Usage and Biology===
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We designed this part in order to validate our newly designed promoter PdtxR (BBa_K2486023).
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The biosensor was able to respond to different concentrations of iron, which suggests it could be used to detect and differentiate Fe<sup>2+</sup> content of the blood.
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====Validation====
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[[File:T--USP-Brazil--Detecttion_Iron_Lactate_dtxR(1).png|thumb|center|500px|Promoter activity (GFP/OD600) under different concentrations of Iron Chloride (Fe<sup>2+</sup>).]]
  
 
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Revision as of 05:06, 1 November 2017


PdtxR/DtxR based Fe2+ biosensor

Biosensor constructed by the ligation of parts BBa_K2486021 and BBa_K2486004.

Usage and Biology

We designed this part in order to validate our newly designed promoter PdtxR (BBa_K2486023). The biosensor was able to respond to different concentrations of iron, which suggests it could be used to detect and differentiate Fe2+ content of the blood.

Validation

File:T--USP-Brazil--Detecttion Iron Lactate dtxR(1).png
Promoter activity (GFP/OD600) under different concentrations of Iron Chloride (Fe2+).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 843
    Illegal NheI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1420
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716