Revision as of 15:26, 30 October 2017

Plasmid partitioning system (SynORI framework)

This particular biobrick encodes low-copy plasmid partitioning system, designed specifically to stabilize SynORI (multi-plasmid system, read more here <link>) low copy plasmid groups, but can also be used to stabilize any type of plasmid.

Sequence and Features

Introduction

Biology

Par region derived from pSC101 is a lot different from its counterparts, for example F and P1 plasmids (Meacock and Cohen 1980). It does not seem to encode any protein but contains binding site for DNA gyrase (Wahle and Kornberg 1988). In contrast, both F and P1 systems encode partitioning proteins in plasmids making Par regions as big as 2kb.

Plasmids with partial deletions of par in pSC101 have decreased negative supercoiling and are extremely unstable (lost from cells in a short amount of time). This has led to proposal that gyrase-generated negative supercoiling establishes a DNA conformation which enables plasmids to interact with E. Coli structures which distribute them to daughter cells at division (Miller et al., 1990).

Usage with SynORI (Framework for multi-plasmid systems)

About SynORI

SynORI is a framework for multi-plasmid systems created by Vilnius-Lithuania 2017 which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!

Active segregation system in SynORI

SynORI system can be used with low plasmid systems, but in order to efficiently run that kind of system, it must have a mechanism to keep low copy plasmids in the cell.

Characterization of Active segregation system (Vilnius-Lithuania 2017)

To be updated!

References