Difference between revisions of "Part:BBa K2201243"

Revision as of 19:45, 24 October 2017

Fusion protein of CFP and YFP with an amber codon in the linker

This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, BBa_K1416004) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.

Important: This part does not contain a promoter! For the functional part and the results, look up to BBa_K2201343.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 718
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1308
Illegal AgeI site found at 1420
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2201243 short</partinfo>
-This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, <b>BBa_K1416004</b>) of the iGEM-Team Austin, Texas, 2014. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.
+This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, <b>BBa_K1416004</b>) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.
 <b>Important:</b> This part does not contain a promoter! For the functional part and the results, look up to <b>BBa_K2201343</b>.