Difference between revisions of "Part:BBa K2201220"

Revision as of 10:22, 25 October 2017

CDS of GFP-streptavidin fusion protein with medium gly-gly-ser linker

This fusion protein containing GFP and streptavidin connected by a Gly-Gly-Ser-linker is used to show the properties and uses of the ncAA 2-NPA. The streptavidin of this fusion protein is able to bind biotin, such that the protein could be immobilized on any biotinylated surface. The GFP is fluorescent, such that the binding of the protein can easily be detected. This version of the fusion protein is needed to validate the binding and detection properties without the incorporation of the ncAA 2-NPA compared to the part K2201221. Experiments with this protein can deliver the reference values of binding efficiency and fluorescence signal undisturbed by the ncAA. When compared to the values of the amber variant, it can be determined if there is any loss or change in the binding or in the fluorescence properties caused by the 2-NPA or the synthetase.

Important: This part only contains the coding sequence and will not be expressed if transformed. For any further results look up to BBa_K2201320.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 787
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 829
Illegal AgeI site found at 880
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2201220 short</partinfo>
-This fusion protein containing GFP and streptavidin connected by a Gly-Gly-Ser-linker is used to show the properties and uses of the ncAA 2-NPA. The streptavidin of this fusion protein is able to bind biotin, such that the protein could be immobilized on any biotinylated surface. The GFP is fluorescent, such that the binding of the protein can easily be detected. This version of the fusion protein is needed to validate the binding and detection properties without the incorporation of the ncAA 2-NPA compared to the part K2201221. Experiments with this protein can deliver the reference values of binding efficiency and fluorescence signal undisturbed by the ncAA. When compared to the values of the amber variant, it can be determined if there is any loss or change in the binding or in the fluorescence properties caused by the 2-NPA or the synthetase.
+This fusion protein containing GFP and streptavidin connected by a Gly-Gly-Ser-linker is used to show the properties and uses of the ncAA 2-NPA. The streptavidin of this fusion protein is able to bind biotin, such that the protein could be immobilized on any biotinylated surface. The GFP is fluorescent, such that the binding of the protein can easily be detected. This version of the fusion protein is needed to validate the binding and detection properties without the incorporation of the ncAA 2-NPA compared to the part [https://parts.igem.org/Part:BBa_BBa_K2201221 K2201221]. Experiments with this protein can deliver the reference values of binding efficiency and fluorescence signal undisturbed by the ncAA. When compared to the values of the amber variant, it can be determined if there is any loss or change in the binding or in the fluorescence properties caused by the 2-NPA or the synthetase.
-<b>Important:</b> This part only contains the coding sequence and will not be expressed if transformed. For any further results look up to BBa_K2201320.
+<b>Important:</b> This part only contains the coding sequence and will not be expressed if transformed. For any further results look up to [https://parts.igem.org/Part:BBa_BBa_K2201320 BBa_K2201320].
 <!-- Add more about the biology of this part here