Difference between revisions of "Part:BBa K2450301"
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<partinfo>BBa_K2450301 short</partinfo>
 
<partinfo>BBa_K2450301 short</partinfo>
  
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This part was designed to test the efficacy of a modified TetR (Tet repressor). It is a medium strength pTet promotor, an RBS, and the coding sequence for eYFP.
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A pTet promoter and RBS encoding for YFP.
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 +
This part was designed to test the efficacy of a modified TetR (Tet repressor), BBa_K2450201. It is a medium strength pTet promotor.
 +
 
 +
This part will work as a reporter for a system relying on a change in the levels of TetR repression.
  
 
===Sequence and Features===
 
===Sequence and Features===
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===Usage and Biology===
 
===Usage and Biology===
  
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pTet is the promoter that can be bound by the Tet repressor, a dimer that is prevented from binding DNA by the presence of the antimicrobial tetracycline (ATC). TetR is a very tight-binding repressor, meaning that leakage is unlikely (in contrast to a system such as Lac).
  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2450301 parameters</partinfo>
 
<partinfo>BBa_K2450301 parameters</partinfo>
 
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Revision as of 19:08, 24 October 2017


pTet promoter coding eYFP

A pTet promoter and RBS encoding for YFP.

This part was designed to test the efficacy of a modified TetR (Tet repressor), BBa_K2450201. It is a medium strength pTet promotor.

This part will work as a reporter for a system relying on a change in the levels of TetR repression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

pTet is the promoter that can be bound by the Tet repressor, a dimer that is prevented from binding DNA by the presence of the antimicrobial tetracycline (ATC). TetR is a very tight-binding repressor, meaning that leakage is unlikely (in contrast to a system such as Lac).

Functional Parameters