Difference between revisions of "Part:BBa K2273000:Design"

Revision as of 16:59, 24 October 2017

P_srfA: a ComA-dependent promoter of Bacillus subtilis

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the srfA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The srfA promoter of B. subtilis was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

PsrfA fwd RFC10	gatcGAATTCGCGGCCGCTTCTAGAGTTGCGGCATCCCGCAAAAAATATTG
PsrfA rev RFC10	gatcCTGCAGCGGCCGCTACTAGTACTTTATAAGCAGTGAACATG

References

Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R (2016) Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production. Journal of Molecular Biology 362: 393-402. PubMed

Jan Maarten van Dijl and Michael Hecker "Bacillus subtilis: from soil bacterium to super-secreting cell factory" Microbial Cell Factories 12:3 (2013): 1-6. PubMed

Ling lin Fu, Zi Rong Xu, Wei Fen Li, Jiang Bing Shuai, Ping Lu, Chun Xia Hu "Protein secretion pathways in Bacillus subtilis: Implication for optimization heterologous protein secretion" Biotechnology Advances 25 (2007): 1-12. PubMed

DOI:10.1016/j.jbiotec.2016.03.002
PMCID:PMC208261
DOI: 10.1093/nar/gkv1242
DOI: 10.1099/mic.0.26003-0
DOI: 10.1002/bit.24524

@@ Line 19: / Line 19: @@
 ===References===
-Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R <i>Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production.</i> Journal of Molecular Biology 362: 393-402. [https://www.ncbi.nlm.nih.gov/pubmed/16930615 PubMed]<br><br>
+Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R (2016) <i>Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production.</i> Journal of Molecular Biology 362: 393-402. [https://www.ncbi.nlm.nih.gov/pubmed/?term=Substitution%20of%20the%20Native%20srfA%20Promoter%20by%20Constitutive%20Pveg%20in%20Two%20B.%20subtilis%20Strains%20and%20Evaluation%20of%20the%20Effect%20on%20Surfactin%20Production PubMed]<br><br>
 Jan Maarten van Dijl and Michael Hecker "<i>Bacillus subtilis</i>: from soil bacterium to super-secreting cell factory" Microbial Cell Factories 12:3 (2013): 1-6. [https://www.ncbi.nlm.nih.gov/pubmed/23311580 PubMed]<br><br>
 Ling lin Fu, Zi Rong Xu, Wei Fen Li, Jiang Bing Shuai, Ping Lu, Chun Xia Hu "Protein secretion pathways in <i>Bacillus subtilis</i>: Implication for optimization heterologous protein secretion" Biotechnology Advances 25 (2007): 1-12. [https://www.ncbi.nlm.nih.gov/pubmed/16997527 PubMed]