Difference between revisions of "Part:BBa K124002"
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Author: Yaakov Bulka
 
Author: Yaakov Bulka
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Summary: we added information about the promoter and on its mechanism.
  
  

Revision as of 21:18, 24 October 2017

Yeast GPD (TDH3) Promoter

Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Group: Tel-Hai 2017
Author: Yaakov Bulka
Summary: we added information about the promoter and on its mechanism.


This is a strong constitutive yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase. Also called TDH3 or GAPDH. Studies have found the promoter activity of TDH3 decreased significantly when glycerol or xylose was supplied as the carbon source and at high temperature (42 °C) . Oxygen conditions had non-significant effect.

About the protein - the promoter was taken from the Glyceraldehyde-3-phosphate dehydrogenase peptide (GAPDH), that involves in glycolysis and gluconeogenesis. The protein is a tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate, detected in the cytoplasm and cell wall. GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria.


http://onlinelibrary.wiley.com/doi/10.1002/yea.1806/full#bib17

https://www.ncbi.nlm.nih.gov/pubmed/26164057