Difference between revisions of "Part:BBa K2371012"
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This chromoprotein eforRed naturally exhibits red/pink color when expressed. The color is slightly weaker than RFP. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation. | This chromoprotein eforRed naturally exhibits red/pink color when expressed. The color is slightly weaker than RFP. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation. | ||
| + | <h5>Figure1.PCR test of eforRed and GFP.</h5> | ||
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| + | [[File:Pcr test of eforRed and GFP.png|400px]] | ||
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| + | <h5>Figure2.RD test of eforRed and GFP.</h5> | ||
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| + | [[File:RD test of amliRed and GFP.png|400px]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:44, 31 October 2017
pT7-amilGFP
This chromoprotein eforRed naturally exhibits red/pink color when expressed. The color is slightly weaker than RFP. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
Figure1.PCR test of eforRed and GFP.
Figure2.RD test of eforRed and GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 600
Illegal AgeI site found at 712 - 1000COMPATIBLE WITH RFC[1000]