Difference between revisions of "Part:BBa K2319002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | <p>Serine, like threonine, has a hydroxyl group that contributes to stability by interacting with the aqueous cellular environment. Glycine, again due to its small size, maintains flexibility of the linker.</p> | ||
| − | + | <p>This linker region also contains a BamHI restriction site (G\GATCC) whose in-frame translation is Gly-Ser. This BamHI site is used to link our two protein domains during assembly of the fusion construct.</p> | |
===Source=== | ===Source=== | ||
Revision as of 10:08, 1 November 2017
GGSGSGSS linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 10
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Serine, like threonine, has a hydroxyl group that contributes to stability by interacting with the aqueous cellular environment. Glycine, again due to its small size, maintains flexibility of the linker.
This linker region also contains a BamHI restriction site (G\GATCC) whose in-frame translation is Gly-Ser. This BamHI site is used to link our two protein domains during assembly of the fusion construct.