Difference between revisions of "Part:BBa K2276007:Experience"

 
(→‎Applications of BBa_K2276007)
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===Applications of BBa_K2276007===
 
===Applications of BBa_K2276007===
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We constructed strains that contain Part:BBa K2276007 and measure the fluorescence intensity and OD600 changes of it.Due to the limit of the expriment condition, we measure the change at the first 8hours and 10~16 hours after inoculated separately.Here is the result.
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[[File:SCU-China 2017 RBS fluorescence intensity over time .png|420px|thumb|right]]
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Figure 1 The fluorescence intensity changes over time(0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.
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[[File:SCU-China 2017 RBS fluorescence intensity over time.png|420px|thumb|right]]
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Figure 2 The OD600 changes over time(0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.
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    We can see that Part:BBa K2276007 has a much strong expression than Part:BBa_I13521.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 08:42, 30 October 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2276007

We constructed strains that contain Part:BBa K2276007 and measure the fluorescence intensity and OD600 changes of it.Due to the limit of the expriment condition, we measure the change at the first 8hours and 10~16 hours after inoculated separately.Here is the result.
File:SCU-China 2017 RBS fluorescence intensity over time .png
420px

Figure 1 The fluorescence intensity changes over time(0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.

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Figure 2 The OD600 changes over time(0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521. 

   We can see that Part:BBa K2276007 has a much strong expression than Part:BBa_I13521.

User Reviews

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