Difference between revisions of "Part:BBa K2224001:Experience"

Revision as of 03:30, 28 October 2017

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2224001

Part Description:

BBa_K2224001 is a composite part with the backbone pSB1C3. The CDS of this part is a chitinase originate from verticillium lecanii, a kind of entomogenous fungus. With a GeneBank identifier DQ412944 in NCBI. This enzyme helps catalyze chitin. SMS students optimized the codons and removed inrtons to fit the original sequence into E. coli expression system.

Experiment Abstract

The original sequence DQ412944 was published on NCPI. SMS students have processed the CDS. Designed primer with restriction sites, modified the CDS for standardization. We then synthesized this plasmid with the backbone pSB1C3. Enzyme activity in TOP10 E. coli expression system related to time shown below.

User Reviews

UNIQf9278c08f378bc8a-partinfo-00000000-QINU UNIQf9278c08f378bc8a-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K2224001===
+====Part Description:====
+	BBa_K2224001 is a composite part with the backbone pSB1C3. The CDS of this part is a chitinase originate from verticillium lecanii, a kind of entomogenous fungus. With a GeneBank identifier DQ412944 in NCBI. This enzyme helps catalyze chitin. SMS students optimized the codons and removed inrtons to fit the original sequence into E. coli expression system.
+====Experiment Abstract====
+	The original sequence DQ412944 was published on NCPI. SMS students have processed the CDS. Designed primer with restriction sites, modified the CDS for standardization. We then synthesized this plasmid with the backbone pSB1C3.
+Enzyme activity in TOP10 E. coli expression system related to time shown below.
+[[File:Chitinase Activity.png]]
 ===User Reviews===