Difference between revisions of "Part:BBa K2368023"
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<h1>Introduction</h1> | <h1>Introduction</h1> | ||
<partinfo>BBa_K2368023 short</partinfo> | <partinfo>BBa_K2368023 short</partinfo> | ||
| − | <p> This part sweetness receptor T1R2 fusion with the | + | <p> This part sweetness receptor T1R2 fusion with the myc tag, just like the picture showed below. </p> |
| − | </p> | + | ] |
| − | + | [[File:T-BIT-China-2017parts-40.png|center|500px|默认文字]] | |
| − | <p> Fig.1 The schematic diagram of | + | <p style="text-align: center">Fig.1 The schematic diagram of myc+T1R2 overlap</p> |
| + | |||
| + | |||
<h1>Design</h1> | <h1>Design</h1> | ||
| − | <p> In order to show whether the sweet receptor T1R2 are translated and located correctly, we fusion the | + | <p> In order to show whether the sweet receptor T1R2 are translated and located correctly, we fusion the myc tag to N-terminal of T1R2. The reason fused tag to N-terminal is that the C-terminal of T1R2 interacts with the G protein α subunit, which is a crucial factor during signal deliver through G protein signal pathway. And the N-terminal is outside the cell, so it can be detected without killing it, using immunofluorescence can figure out whether T1R2 is located at the right position. </p> |
| − | + | ||
| − | <p> Fig.2 Electrophoresis of | + | |
| + | [[File:T-BIT-China-2017parts-41.png|center|500px|默认文字]] | ||
| + | <p style="text-align: center">Fig.2 Electrophoresis of myc+T1R2 overlap. </p> | ||
| + | |||
<h1>Experiment</h1> | <h1>Experiment</h1> | ||
| − | <p> At the beginning, we construct the specific primer that consists of the gene sequence of | + | <p> At the beginning, we construct the specific primer that consists of the gene sequence of myc tag. Then, fused T1R2 with the myc using PCR. The length of sequence is 517 bp. </p> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<h2>Sequence and Features</h2> | <h2>Sequence and Features</h2> | ||
| − | <partinfo> | + | <partinfo>BBa_K2368009 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
| − | <partinfo> | + | <partinfo>BBa_K2368009 parameters</partinfo> |
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Revision as of 18:13, 25 October 2017
Introduction
Myc+T1R2 overlap
This part sweetness receptor T1R2 fusion with the myc tag, just like the picture showed below.
]
Fig.1 The schematic diagram of myc+T1R2 overlap
Design
In order to show whether the sweet receptor T1R2 are translated and located correctly, we fusion the myc tag to N-terminal of T1R2. The reason fused tag to N-terminal is that the C-terminal of T1R2 interacts with the G protein α subunit, which is a crucial factor during signal deliver through G protein signal pathway. And the N-terminal is outside the cell, so it can be detected without killing it, using immunofluorescence can figure out whether T1R2 is located at the right position.
Fig.2 Electrophoresis of myc+T1R2 overlap.
Experiment
At the beginning, we construct the specific primer that consists of the gene sequence of myc tag. Then, fused T1R2 with the myc using PCR. The length of sequence is 517 bp.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]