Difference between revisions of "Part:BBa K2368020"
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__NOTOC__
 
__NOTOC__
 
<h1>Introduction</h1>
 
<h1>Introduction</h1>
 
<partinfo>BBa_K2368020 short</partinfo>
 
<partinfo>BBa_K2368020 short</partinfo>
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<p>This part is the overlap of that sweetness receptor T1R3 combines with the BCP tag. </p>
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<p> This part is the overlap of that sweetness receptor T1R3 combines with the bcp tag. </p>
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<p>The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal transduction through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and BCP tag.</P>
+
<p> The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and bcp tag.</P>
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<P>The result of the part is that the expression of the blue cyanidin protein to detect the expression and the location of the T1R3.</p>
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<P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.</p>
 
<h1>Design</h1>
 
<h1>Design</h1>
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<p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and BCP. Then, we combined the overlap of T1R3 with the BCP by PCR.</p>
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<p> Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and bcp. Then, we combined the overlap of T1R3 with the bcp by PCR.</p>
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https://static.igem.org/mediawiki/2017/f/f3/BCP3-1.png
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[[File:T-BIT-China-2017parts-30.png|center|500px|默认文字]]
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<p>Fig.1 The schematic diagram of BCP+T1R3 overlap</p>
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<p style="text-align: center">Fig.1 The schematic diagram of bcp+T1R3 overlap</p>
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[[File:T-BIT-China-2017parts-31.png|center|500px|默认文字]]
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<p style="text-align: center">Fig. 2 The PCR method of bcp+T1R3 overlap</p>
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<h1>Experiment</h1>
 
<h1>Experiment</h1>
 
<p>This part is used to detect the expression and the location of the T1R3.</p>
 
<p>This part is used to detect the expression and the location of the T1R3.</p>
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https://static.igem.org/mediawiki/2017/a/a9/BCP3-2.png
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[[File:T-BIT-China-2017parts-32.png|center|500px|默认文字]]
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<p>Fig.2 Electrophoresis of BCP+T1R3 overlap</p>
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<p style="text-align: center">Fig.3 Electrophoresis of bcp+T1R3 overlap. </p>
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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<h2>Sequence and Features</h2>
 
<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368020 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2368013 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2368020 parameters</partinfo>
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<partinfo>BBa_K2368013 parameters</partinfo>
 
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Revision as of 17:39, 25 October 2017


Introduction

BCP+T1R3 overlap

This part is the overlap of that sweetness receptor T1R3 combines with the bcp tag.

The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and bcp tag.

The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.

Design

Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and bcp. Then, we combined the overlap of T1R3 with the bcp by PCR.

默认文字

Fig.1 The schematic diagram of bcp+T1R3 overlap

默认文字

Fig. 2 The PCR method of bcp+T1R3 overlap

Experiment

This part is used to detect the expression and the location of the T1R3.

默认文字

Fig.3 Electrophoresis of bcp+T1R3 overlap.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]