Difference between revisions of "Part:BBa K2194002:Design"
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+ | We sourced the nucleotide sequence for <i>Pgntk</i> from the <i>Escherichia coli</i> BW25113 genome NCBI database (entry CP009273.1) by extracting the 200 bp uspstream of the <i>gntK</i> gene. This range was based on previous research that demonstrated that this region contained the full promoter sequence because the same negative feedback response was observed whether using 500 or 200 bp upstream of <i>gntK</i> as the promoter. [2] We then sent this sequence to IDT to manufacture as a gBlock®. | ||
===References=== | ===References=== |
Revision as of 05:44, 1 November 2017
PgntK Negative Feedback Membrane Stress Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 14
Illegal BsaI.rc site found at 216
Design Notes
[update]
Source
We sourced the nucleotide sequence for Pgntk from the Escherichia coli BW25113 genome NCBI database (entry CP009273.1) by extracting the 200 bp uspstream of the gntK gene. This range was based on previous research that demonstrated that this region contained the full promoter sequence because the same negative feedback response was observed whether using 500 or 200 bp upstream of gntK as the promoter. [2] We then sent this sequence to IDT to manufacture as a gBlock®.