Difference between revisions of "Part:BBa K2194001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Primers used to PCR amplify <i>sbp</i> from the <i>Escherichia coli</i> MG1655 genome<sup>NOTE</sup>: <p> | |
− | + | 5' <b>CGATCGTCTCACTCGA</b>ATGAACAAGTGGGGCGTAG3'</p><p> | |
+ | 5' <b>GCATCGTCTCACTCTGCCA</b>TCAGCGTTTGCTGATCTG3'</p> | ||
+ | <br> | ||
+ | NOTE: The primer overhangs are <b>bolded</b>. | ||
===Source=== | ===Source=== |
Revision as of 02:40, 1 November 2017
Sulfate Binding Protein (sbp) cds
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 1006
Design Notes
Primers used to PCR amplify sbp from the Escherichia coli MG1655 genomeNOTE:5' CGATCGTCTCACTCGAATGAACAAGTGGGGCGTAG3'
5' GCATCGTCTCACTCTGCCATCAGCGTTTGCTGATCTG3'
NOTE: The primer overhangs are bolded.
Source
Escherichia coli genomic sequence