Difference between revisions of "Part:BBa K2332000:Design"

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SpyCatcher sequence was obtained from [https://parts.igem.org/Part:BBa_K1159200 BBa_K1159200] and GFP from:  
 
SpyCatcher sequence was obtained from [https://parts.igem.org/Part:BBa_K1159200 BBa_K1159200] and GFP from:  
−
[https://parts.igem.org/Part:BBa_E0040 BBa_E0040]. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
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[https://parts.igem.org/Part:BBa_E0040 BBa_E0040]. Pblind promoter was designed by Jayaraman P. et al. (2016). The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
  
 
===References===
 
===References===

Revision as of 23:32, 23 October 2017


GFP-SpyTag (constitutive)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.


Source

SpyCatcher sequence was obtained from BBa_K1159200 and GFP from: BBa_E0040. Pblind promoter was designed by Jayaraman P. et al. (2016). The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References