Difference between revisions of "Part:BBa K2429019:Design"
(→Design Notes) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | The second HBG intron has a point mutation at bp 654 to match the intron used in a paper that used the HBG intron as part of their reporter. | + | This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. The second HBG intron has a point mutation at bp 654 to match the intron used in a paper that used the HBG intron as part of their reporter. |
| − | + | ||
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 14:59, 26 October 2017
3 Exon eYFP Reporter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2282
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2282
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2282
Illegal BamHI site found at 1
Illegal BamHI site found at 1412 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2282
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2282
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. The second HBG intron has a point mutation at bp 654 to match the intron used in a paper that used the HBG intron as part of their reporter.
Source
The human beta globin introns came from the HEK cell genome. The sequences for YFP and BFP were obtained through BLAST, and the part comes from the genome of a jellyfish.