Difference between revisions of "Part:BBa K2217027"
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<partinfo>BBa_K2217027 short</partinfo>
 
<partinfo>BBa_K2217027 short</partinfo>
  
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laci registered at [https://parts.igem.org/Part:BBa_K2217012# (BBa_K2217012)], was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding.
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==Egypt-AFCM Team Improvement==
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[http://2017.igem.org/Design.html# Egypt-AFCM Team] tried to improve this part's function [BBa K1179062] regarding inhibition of non-coding RNAs by fusing laci  the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site.  Part characterization and usage can be found at  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2217027# BBa_K2217027_Experience].
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Revision as of 17:39, 24 October 2017


laci + miRNA-1825 binding site synthesized fragment

Egypt-AFCM Team Improvement

[http://2017.igem.org/Design.html# Egypt-AFCM Team] tried to improve this part's function [BBa K1179062] regarding inhibition of non-coding RNAs by fusing laci the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site. Part characterization and usage can be found at BBa_K2217027_Experience.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]