Difference between revisions of "Part:BBa K2217027"
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laci registered at [https://parts.igem.org/Part:BBa_K2217012# (BBa_K2217012)], was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding. | laci registered at [https://parts.igem.org/Part:BBa_K2217012# (BBa_K2217012)], was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding. | ||
| + | This part was designed in a trail to induce the expression of [https://parts.igem.org/Part:BBa_K2217001# Cirular-RNA hsa_circ_0000064] when ever the miRNA binding site fused to laci senses high binding rate of miRNA-1825. | ||
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==Usage and Biology== | ==Usage and Biology== | ||
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Revision as of 16:25, 23 October 2017
laci + miRNA-1825 binding site synthesized fragment
laci registered at (BBa_K2217012), was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding. This part was designed in a trail to induce the expression of Cirular-RNA hsa_circ_0000064 when ever the miRNA binding site fused to laci senses high binding rate of miRNA-1825.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]