Difference between revisions of "Part:BBa K2316000"
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<partinfo>BBa_K2316000 short</partinfo> | <partinfo>BBa_K2316000 short</partinfo> | ||
− | + | dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner. This is an improvement from part BBa_K2130000 | |
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Revision as of 04:36, 25 October 2017
∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9
dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner. This is an improvement from part BBa_K2130000
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 804
Illegal BamHI site found at 1622
Illegal XhoI site found at 2578 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1966
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2112
Illegal BsaI site found at 2774
Illegal BsaI.rc site found at 1027