Difference between revisions of "Part:BBa K2371008:Design"

 
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===Design Notes===
 
===Design Notes===
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This coding sequence consists of the alternative target sequence of 70B and the scaffold sequence. The target sequence is complementary to one of the sites of the fusion gene EML4-ALK.
 
  
  
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===Source===
 
===Source===
  
−
DNAworks sequence synthesis
+
The EML4-ALK fused gene has two variants (A and B).
 +
 
 +
We obtained the sequence of EML4-ALK from NCBI.
 +
 
 +
https://www.ncbi.nlm.nih.gov/nuccore/AB374361.1 EML4-ALK variant 3 a
 +
 
 +
https://www.ncbi.nlm.nih.gov/nuccore/AB374362.1 EML4-ALK variant 3 b
 +
 
 +
The part was synthesized by SOEPCR designed by DNAworks.
  
 
===References===
 
===References===
 +
1. Wong DW, et al. The EML4-ALK fusion gene is involved in various histologic types of lung cancers from nonsmokers with wild-type EGFR and KRAS. Cancer. 2009 Apr 15;115(8):1723-33.

Revision as of 07:56, 31 October 2017


sgRNA generator for EML4-ALK variant B 70


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The EML4-ALK fused gene has two variants (A and B).

We obtained the sequence of EML4-ALK from NCBI.

https://www.ncbi.nlm.nih.gov/nuccore/AB374361.1 EML4-ALK variant 3 a

https://www.ncbi.nlm.nih.gov/nuccore/AB374362.1 EML4-ALK variant 3 b

The part was synthesized by SOEPCR designed by DNAworks.

References

1. Wong DW, et al. The EML4-ALK fusion gene is involved in various histologic types of lung cancers from nonsmokers with wild-type EGFR and KRAS. Cancer. 2009 Apr 15;115(8):1723-33.