Difference between revisions of "Part:BBa K2505004"
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| − | + | ==Characterization== | |
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| + | ===Sub part=== | ||
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| + | <!-- Add more about the biology of this part here | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K2505003 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K2505003 parameters</partinfo> | ||
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Revision as of 07:42, 23 October 2017
atipt4-IVS-IRES-log1-polyA
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1193
Illegal XhoI site found at 2243
Illegal XhoI site found at 2255 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 739
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 881
This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP(isopentenyl adenine: kind of cytokinin) as a signaling molecule. atipt4 gene and log1 gene is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase [EC:2.5.1.112]: cytokinin synthase) that necessary for a synthesis of iP. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.
Contents
Characterization
Sub part
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 225
Illegal XhoI site found at 1275
Illegal XhoI site found at 1287 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]