Difference between revisions of "Part:BBa K2247000"

 
Line 3: Line 3:
 
<partinfo>BBa_K2247000 short</partinfo>
 
<partinfo>BBa_K2247000 short</partinfo>
  
−
This is a modified version of the yeast pGal promoter, from which expression can be induced by galactose, or the yeast Gal4 activator.
+
This is a modified version of the yeast endogenous Gal1 promoter, from which expression can be induced by galactose, or the yeast Gal4 activator. pGalS also contains the upstream activating sequence (UAS) which Gal4 can bind, and can serve as the promoter in a standard yeast-two-hybrid system.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:14, 26 October 2017


pGalS promoter of S. cerevisiae

This is a modified version of the yeast endogenous Gal1 promoter, from which expression can be induced by galactose, or the yeast Gal4 activator. pGalS also contains the upstream activating sequence (UAS) which Gal4 can bind, and can serve as the promoter in a standard yeast-two-hybrid system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]