Difference between revisions of "Part:BBa K2368028"

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__NOTOC__
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<h1>Introduction</h1>
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<partinfo>BBa_K2368028 ort</partinfo>
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<p> Gα plays an important role in<i> Saccharomyces Cerevisiae CEN.PK2-1C </i>MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream. </p>
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<h1>Design</h1>
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<p> Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.</p>
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https://static.igem.org/mediawiki/2017/4/41/G-1.png 
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<p> Fig.1 The schematic diagram of Modified Gα</p>
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<h1>Experiment</h1>
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<p> The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.</p>
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https://static.igem.org/mediawiki/2017/1/18/G-2.png 
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<p> The electrophoresis of the positive result of Gα.</p>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368028 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2368028 arameters</partinfo>
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Revision as of 03:22, 24 October 2017


Introduction

BBa_K2368028 ort Not understood

Gα plays an important role in Saccharomyces Cerevisiae CEN.PK2-1C MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream.

Design

Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.

G-1.png

Fig.1 The schematic diagram of Modified Gα

Experiment

The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.

G-2.png

The electrophoresis of the positive result of Gα.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]