Difference between revisions of "Part:BBa K2244004"
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This part is a coding gene mheI (for MBC-hydrolyzing enzyme) that encodes MBC-hydrolyzing esterase (MHE) to degrade MBC pesticide. Degradation of MBC is achieved by hydrolyzing MBC to 2-aminobenzimidazole (2-AB), which is then converted to benzimidazole or 2-hydroxybenzimidazole (2-HB). The conversion of 2-AB was inhibited by NH4NO3. The benzene ring of 2-HB was further opened through meta catechol cleavage. MHE is responsible for carrying out the first step detoxification (MBC-2-AB), without the need of any cofactor. MHE is a soluble serine hydrolase of 242 amino acid residues and has a molecular size of 25-27 kDa. | This part is a coding gene mheI (for MBC-hydrolyzing enzyme) that encodes MBC-hydrolyzing esterase (MHE) to degrade MBC pesticide. Degradation of MBC is achieved by hydrolyzing MBC to 2-aminobenzimidazole (2-AB), which is then converted to benzimidazole or 2-hydroxybenzimidazole (2-HB). The conversion of 2-AB was inhibited by NH4NO3. The benzene ring of 2-HB was further opened through meta catechol cleavage. MHE is responsible for carrying out the first step detoxification (MBC-2-AB), without the need of any cofactor. MHE is a soluble serine hydrolase of 242 amino acid residues and has a molecular size of 25-27 kDa. | ||
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Revision as of 05:44, 23 October 2017
mheI
This part is a coding gene mheI (for MBC-hydrolyzing enzyme) that encodes MBC-hydrolyzing esterase (MHE) to degrade MBC pesticide. Degradation of MBC is achieved by hydrolyzing MBC to 2-aminobenzimidazole (2-AB), which is then converted to benzimidazole or 2-hydroxybenzimidazole (2-HB). The conversion of 2-AB was inhibited by NH4NO3. The benzene ring of 2-HB was further opened through meta catechol cleavage. MHE is responsible for carrying out the first step detoxification (MBC-2-AB), without the need of any cofactor. MHE is a soluble serine hydrolase of 242 amino acid residues and has a molecular size of 25-27 kDa.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 591
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 250
- 1000COMPATIBLE WITH RFC[1000]