Difference between revisions of "Part:BBa K2314212"

Revision as of 00:32, 23 October 2017

UTRrpsT enhanced promoter

UTRrpsT by combining with J23108, to achieve the effect of enhancing J23108. This part contains J23108, UTRrpsT and RBS. UTRrpsT has the ability to enhance the promoter. If you need to enhance the expression of a promoter, you can add UTRrpsT way to achieve. The reason why UTRrpsT is not a separate part is the possibility that the prefix and suffix sequences form a special secondary structure on the 5'UTR. For more details you can view the papers.In short，it can be used as a combination of a promoter and a RBS.

This is the structure of this part.

<img src=""/> Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 56
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 UTRrpsT by combining with J23108, to achieve the effect of enhancing J23108. This part contains J23108, UTRrpsT and RBS. UTRrpsT has the ability to enhance the promoter. If you need to enhance the expression of a promoter, you can add UTRrpsT way to achieve. The reason why UTRrpsT is not a separate part is the possibility that the prefix and suffix sequences form a special secondary structure on the 5'UTR. For more details you can view the papers.In short&#65292;it can be used as a combination of a promoter and a RBS.
+This is the structure of this part.
 <img src="https://static.igem.org/mediawiki/parts/2/25/T--OUC-China-utrrbs.png"/>